Rat PUMA (P53 Upregulated Modulator of Apoptosis) ELISA Kit
SKU: 93912349548

Rat PUMA (P53 Upregulated Modulator of Apoptosis) ELISA Kit

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Description

Rat PUMA (P53 Upregulated Modulator of Apoptosis) ELISA KitThe Rat PUMA (P53 Upregulated Modulator of Apoptosis) ELISA Kit measures PUMA in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PUMA antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Rat PUMA (P53 Upregulated Modulator of Apoptosis) ELISA Kit measures PUMA in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PUMA antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with PUMA bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of PUMA in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 93912349548

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4.6 ★★★★★
Based on 1525 reviews
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M
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MW
Carnegie, US
★★★★★ 1
Squeaker inside toy breaks after 2 days
Size: Mini, Color: Gator (Pink)
Bought for my 8 lb chihuahua for Christmas. I have 3 little girls. The youngest loves her toys and of course she took this one over immediately. She just loved everything about it. The material on the gator is amazingly durable and I believe would hold up for a good while. The squeaker inside the toy however, broke after 2 days of my girl playing with it. It's now a noiseless toy that she does not like too much as it does not squeak because it only had the one squeaker in it. I would recommend that 2 squeakers be inserted into this gator. I would not purchase again due to the longevity of the squeaker...only lasted 2 days.
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Reviewed in the United States on January 5, 2026
C
Verified Purchase
Celeste A
Louisville, US
★★★★★ 5
Go dog toys are the best!
Size: Large, Color: Dinos Frills (Pink)
These are the only stuffed toys I buy for my Pittie because they are so well made. Even when she unstuffs them, she plays with them forever!
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Reviewed in the United States on May 16, 2026
A
Verified Purchase
a customer
Whiting, US
★★★★★ 5
My dog loves these toys.
Size: Mini, Color: Gator (Pink)
Not a huge toy, nor is my dog. But we have lots of goDog toys. Practically indestructible and my dog likes to carry these in his mouth and bring them onto the foot of our bed. Brilliant colors and good squeaker but my dog is afraid of the squeak. Fair value for cost.
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Reviewed in the United States on June 10, 2026
U
Verified Purchase
University Doc
Omaha, US
★★★★★ 5
Our XL dogs love them. Highly recommend it!
Size: Mini, Color: Gator (Pink)
Our XL dogs love this! Highly recommend them! Highly recommend. Not for aggressive chewers but our big dogs don't destroy their toys. They cherish them. Great for play and cuddling. Not to loud either. Very cute.
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Reviewed in the United States on May 13, 2026
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Verified Purchase
Valhalla49
Fort Morgan, US
★★★★★ 5
When they said strong...
...they weren't kidding. Our pup would chew through everything she could get her sharks teeth into. Like my arms. Nothing lasted as long as a week or maybe two. But she has had Dino for months now. She loves him. He is the go to toy for when she wants to play tug. The material used to make this toy is every bit as strong as the seller claims. I will definitely buy from them again. And I will not wait for this one to rip. I may be waiting a long time. If your pup likes to tear stuff apart, feel safe in buying this toy, UPDATE: 2/9/2023 Dino finally succumbed to the torture. His back ripped open and despite trying to repair him with ordinary thread he couldn't withstand the pressure, His stuffing was removed and spread all over the living room. Completely emptied he is still a good toy. Strong enough to withstand the forceful play of tug-o-war. Only thing I would suggest is to use something stronger when closing his back. The rest of him is great. I will be replacing him soon.
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Reviewed in the United States on January 30, 2023

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